Journal: bioRxiv
Article Title: Optimising the use of Oxford nanopore sequencing technology for detection of Wolbachia bacterial endosymbionts in Anopheles mosquitoes
doi: 10.1101/2025.06.16.659891
Figure Lengend Snippet: All bar graphs depict sequencing runs using two different barcoding kits: the Rapid PCR Barcoding Kit 24 V14 (runs 2-6) and the 16S Barcoding Kit 24 V14 (runs 7,8,9,11,12). Each run is represented by a vertical column. A ) Total Data generated (GB). B ) Percentage of passed vs failed bases called for each run. C ) Total number of reads obtained in each sequencing run. D ) Percentage of unclassified passed reads and unclassified passed bases for each run.
Article Snippet: Extracted gDNA from individual mosquitoes was prepared by diluting 10ng of DNA in 15μL of nuclease free water and then the diluted DNA was combined with NEB LongAmp Taq 2x master mix and respective 16S barcodes.
Techniques: Sequencing, Generated